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R&D Systems
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Bio-Techne corporation
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Trevigen
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Merck & Co
cultrex reduced growth factor basement membrane extract ![]() Cultrex Reduced Growth Factor Basement Membrane Extract, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/cultrex reduced growth factor basement membrane extract/product/Merck & Co Average 90 stars, based on 1 article reviews
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Fisher Scientific
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Bio-Techne corporation
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Bio-Techne corporation
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RAN biotechnologies
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Fisher Scientific
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Image Search Results
Journal: PLoS ONE
Article Title: Cell Population Kinetics of Collagen Scaffolds in Ex Vivo Oral Wound Repair
doi: 10.1371/journal.pone.0112680
Figure Lengend Snippet: The ex vivo wound healing model is shown in top view and cross-sectional view (A). The bottom of the well was covered with an acellular growth factor reduced basal membrane extract (BME). The scaffold (sponge type scaffold composed of cross-linked collagen or the gel type scaffold composed of rat collagen I) in the center was surrounded by a BME-gel containing 800,000 hGFs/ml and covered with an acellular BME gel and Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 1% fetal bovine serum (FBS) and antibiotics. Both scaffold and gel were tested with and without the addition of platelet-derived growth factor-BB (PDGF). Fluorescence images were taken to assess DiI-labeled cells within the scaffold over time. To assess the metabolic activity and gene expression dynamics, the scaffolds including the “active zone” (dashed line) were subjected to MTT tests, gene array analysis, and quantitative PCR. (B) Representative scanning electron microscopy images depict the morphology of the collagen gel, collagen scaffold at 400×, and 800× magnification. The white bar represents 100 µm.
Article Snippet: 48 well plates were first layered with 50 μL of 12 mg/ml
Techniques: Ex Vivo, Membrane, Modification, Derivative Assay, Fluorescence, Labeling, Activity Assay, Gene Expression, Real-time Polymerase Chain Reaction, Electron Microscopy
Journal: Bio-protocol
Article Title: An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs
doi: 10.21769/BioProtoc.5160
Figure Lengend Snippet: A) Place the core-needle biopsy into 500 μL of digestion solution. B) Slice the biopsy into 1–3 mm pieces. C) Move pieces into a 50 mL tube containing 10 mL of digestion solution. D) Stop digestion when pieces appear smaller and/or the digestion solution becomes “cloudy.” E) Spin down supernatant to pellet fragments. F) Pipette 50 μL of Cultrex and fragment mix into the middle of the well. G) Add PDO media by pipetting to the wall of the well.
Article Snippet:
Techniques: Transferring